For Research Use Only, not for use in diagnostic procedures

UltiMapper I/O T-act

Determine whether T cells have the potential to mediate cell death

This T cell activation 4-plex/5-color kit enables co-expression of proliferating cells, cytotoxic cells, tumor cells, and the underlying proliferative index and potential for T cell mediated death.

This kit contains all reagents ti stain and image:

  • CD3
  • Granzyme B
  • Ki67
  • panCK / SOX10
  • Nuclear counterstain
SKU: Ut-22 Category:

For more information about this product please contact Ultivue EMEA contact.emea@ultivue.com

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For more information about this product please contact our local distributor in Japan, Cosmo Bio
mail@cosmobio.co.jp, +81 3 5632 9610

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For more information about this product please contact our local distributor in South Korea, MDxK
info@mdxk.co.kr, +82 2 578 8848

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For more information about this product please contact our local distributor in Singapore, AITbiotech
sales@aitbiotech.com, +65 6778 6822

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For more information about this product please contact our local distributor in Taiwan, Bond Biotech.
bond.biotech@gmail.com, +886 4 2358 3959

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For more information about this product please contact our local distributor in China, Leica Biosytems China,
+86 400 821 3236

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Features

Cell Phenotypes

Cell Phenotyping with the UltiMapper I/O T-act Kit

For the determination of cell proliferation and T-cell activation

UltiMapper I/O T-act kit(ULT20104)staining non-small cell lung cancer tissue.

UltiMapper I/O T-act kit(ULT20104)staining non-small cell lung cancer tissue. CD3 (red), Granzyme B(green), Ki67(orange), panCK (cyan), and nuclear counterstain (blue).

The antibody panel in the UltiMapper I/O T-act kit enables users to identify activated T cells. CD3 is a marker of T cells. Granzyme B is produced, stored, and released by cytotoxic T cells to mediate cellular death in combination with perforin. Ki67 is a nuclear protein that is a marker of proliferation. Co-expression of CD3, Granzyme B, and Ki67 indicates proliferating cytotoxic T cells. SOX10 is a tumor marker for melanomas while panCK detects carcinomas (provided in a cocktail).

Markers
Phenotype CD3 Granzyme B Ki67 panCK/SOX10
T cells
Cytotoxic cells
Proliferating cells
Carcinoma or Melanoma
Cytotoxic T cells
Proliferating T cells
Proliferating cytotoxic cells
Proliferating cytotoxic T cells
Proliferating tumour cells
Product Biology
Marker Main Cell Type Function
CD3 T cells Identifies all T cells and is the most specific marker for T cells, including lineage based markers such as CD4 and CD8.
Granzyme B Cytotoxic T cells & NK cells Granzyme B is produced, stored, and released by cytotoxic cells to mediate cellular death in combination with Perforin.
Ki67 Proliferating cells Ki67 is expressed within the nucleus during the phases of mitosis and is strongly associated with cell proliferation.
CK/Sox10 Tumor cells The kit may be used to interrogate many tumor sample types for Research. A cocktail of optimized reagents for the detection of pan-Cytokeratin and Sox10 protein markers is provided. Cytokeratins are expressed in cells of an epithelial origin including most carcinomas. Sox10 is expressed in cells derived from the neural crest including melanocytes that give rise to melanomas.

Frequently Asked Questions

Q How many slides can I stain with a single kit?

A

Each UltiMapper kit can stain10 slides when running the assay on the Leica BOND RX. When performing the assay manually, each kit can stain 12+ slides.

Q What image analysis software can I use?

A

Image analysis can be performed on a number of different software platforms, including Indica Labs HALO, Visiopharm, ImageJ, and other platforms that can manage multiple fluorescent channels for analysis.

Q What imaging systems do I need to have?

A

UltiMapper kits are compatible with most fluorescent scanners. However, the imaging systems will need to have the appropriate filter sets including Cy7 capabilities.

Related Products

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The PD-L1 immune infiltration kit enables co-localization of macrophages, cytotoxic T cells, tumor cells, and the underlying inhibitory or inflammatory mechanisms at play along the PD-L1 axis.

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Profile myeloid-derived suppressor cells

The MDSC kit enables the visualization of complex phenotypes through a combination of positive, negative, and range of expression of markers to identify M-MDSCs and PMN-MDSCs.

Looking for a Custom Solution?

If you have specific research needs beyond our standard panel, we accommodate tailored design of custom panels. We are committed to delivering broader tumor immunology profiles, generating critical information to decipher complex mechanisms of cancer development and progression, and identifying relevant signatures of markers for research applications

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InSituPlex

Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

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With speed and flexibility at heart, our Staining & Imaging services and Biomarker Programs offer highly customizable panels to include all your markers of interest.

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Explore our Technology

InSituPlex

Our proprietary InSituPlex technology enables unsurpassed biomarker detection and analysis in tissue, with no compromise on level of multiplexing, throughput of sample staining and imaging, and with no requirement of dedicated instrumentation.

Services

Explore our Services

Services & Programs

With speed and flexibility at heart, our Biomarker Programs offer 5-Plex validated and highly customizable panels to include all your markers of interest.

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